Green tea (Camellia sinensis)
Inhibition by green tea catechins of metabolic activation of procarcinogens by human cytochrome P450
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Inhibition by green tea catechins of metabolic activation of procarcinogens by human cytochrome P450.
1 . Percent inhibition of CYP2E1 by epicatechin gallate (id=NPDI-4i54tA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — not provided
- CYP2E1 4173608
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
30 min at pH 6.8
NADPH regenerating system
Not available
Not available
100 µM
0-100 µM
2 . Unnamed Experiment (id=NPDI-_5Ix4Q)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP2E1 4173608
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
30 min at pH 6.8
NADPH regenerating system
Not available
Not available
100 µM
50 µM
3 . Percent inhibition of CYP1A1 by epicatechin gallate (id=NPDI-X_yVFw)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — not provided
- CYP1A1 4173297
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
15 min
NADPH regenerating system
Not available
Not available
10 µM
0-100 µM
4 . Unnamed Experiment (id=NPDI-vyz23w)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP1A1 4173297
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
15 min
NADPH regenerating system
Not available
Not available
10 µM
50 µM
5 . Percent inhibition of CYP1A2 by epicatechin gallate (id=NPDI-ArhlIA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — not provided
- CYP1A2 4312402
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 40 pmol/mL
In-house preparation
0.5 mL
10 min
NADPH regenerating system
Not available
Not available
1 µM
0-100 µM
6 . Unnamed Experiment (id=NPDI-p7eJTA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP1A2 4312402
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 40 pmol/mL
In-house preparation
0.5 mL
10 min
NADPH regenerating system
Not available
Not available
1 µM
10 µM
7 . Percent inhibition of CYP2A6 by epicatechin gallate (id=NPDI-CPfQsA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — not provided
- CYP2A6 4309826
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
15 min
NADPH regenerating system
Not available
Not available
1 µM
0-100 µM
8 . Unnamed Experiment (id=NPDI-q6-gZQ)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP2A6 4309826
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
15 min
NADPH regenerating system
Not available
Not available
1 µM
50 µM
9 . Percent inhibition of CYP2C9 by epicatechin gallate (id=NPDI-2KGgbg)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — not provided
- CYP2C9 4309227
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.25 mL
15 min
NADPH regenerating system
Not available
Not available
5 µM
0-100 µM
10 . Unnamed Experiment (id=NPDI-38xlLg)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP2C9 4309227
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.25 mL
15 min
NADPH regenerating system
Not available
Not available
5 µM
20 µM
11 . Percent inhibition of CYP3A4 by epicatechin gallate (id=NPDI-JGDRVA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — not provided
- CYP3A4 4306811
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 60 pmol/mL
In-house preparation
0.5 mL
5 min
NADPH regenerating system
Not available
Not available
10 µM
0-100 µM
12 . Unnamed Experiment (id=NPDI-Cuu5WA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP3A4 4306811
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 60 pmol/mL
In-house preparation
0.5 mL
5 min
NADPH regenerating system
Not available
Not available
10 µM
10 µM
13 . Percent inhibition of CYP1A1 by (-)-epicatechin (id=NPDI-pD4STA)
In Vitro Enzyme Inhibition Experiment
Negligible Inhibition was detected. Cutoff used — not provided
- CYP1A1 4173297
Recombinant expression system
Not available
Cytochrome B5 Not available
Results
Percent inhibition at 100 µM as estimated from Fig. 3 is presented.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 20 pmol/mL
In-house preparation
0.5 mL
15 min
NADPH regenerating system
Not available
Not available
10 µM
0-100 µM