[{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2166,"experimentAnswers.text":"10 min","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":6,"experimentAnswers.question.id":6,"experimentAnswers.question.text":"Incubation time","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2167,"experimentAnswers.text":"10 µM","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":11,"experimentAnswers.question.id":11,"experimentAnswers.question.text":"Object concentrations tested","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2168,"experimentAnswers.text":"30% v/v","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":15,"experimentAnswers.question.id":15,"experimentAnswers.question.text":"Precipitant concentrations tested","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2169,"experimentAnswers.text":"Healthya green tea (Kao Corp., Tokyo, Japan)","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":26,"experimentAnswers.question.id":26,"experimentAnswers.question.text":"Precipitant source","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2170,"experimentAnswers.text":"5.0 x 10^5 cells/well","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":33,"experimentAnswers.question.id":33,"experimentAnswers.question.text":"Density","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2171,"experimentAnswers.text":"12-well poly-D-lysine coated plates","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":35,"experimentAnswers.question.id":35,"experimentAnswers.question.text":"Plate type","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2172,"experimentAnswers.text":"72 h","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":36,"experimentAnswers.question.id":36,"experimentAnswers.question.text":"Days after plating","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2173,"experimentAnswers.text":"37 deg C","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":40,"experimentAnswers.question.id":40,"experimentAnswers.question.text":"Incubation temperature","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null},{"id":22,"uid":"NPDI-GdcFMg","name":"Inhibition of MATE2-K by Green Tea","overallEffect":1,"isControlData":false,"isIc50Shift":false,"croCutoff":"&gt;20% inhibition vs. control","croIdentifier":null,"comment":null,"experimentalConditionsComment":"Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.","resultsComment":"Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC<sub>50</sub> value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.","internalComment":null,"objectCompoundId":49,"objectMetaboliteCompoundId":null,"precipitantCompoundId":35,"cytochromeB5Id":null,"studyId":10,"experimentTypeId":5,"testSystemId":22,"ic50ShiftExperimentId":null,"controlDataExperimentId":74,"controlDataForExperimentId":74,"naturalProductSampleId":null,"experimentType.id":5,"experimentType.name":"In Vitro Transporter Inhibition","experimentType.isInVitro":true,"experimentType.isTransporter":true,"experimentType.isEnzyme":false,"experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00000060","objectCompound.id":49,"objectCompound.name":"metformin","objectCompound.unii":null,"objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","objectCompound.publicDescription":null,"objectCompound.internalComment":null,"objectCompound.conceptId":1503297,"objectCompound.enantiomerOfId":null,"objectCompound.concept.conceptId":1503297,"objectCompound.concept.conceptName":"Metformin","objectCompound.concept.domainId":"Drug","objectCompound.concept.vocabularyId":"RxNorm","objectCompound.concept.conceptClassId":"Ingredient","objectCompound.concept.standardConcept":"S","objectCompound.concept.conceptCode":"6809","objectCompound.concept.validStartDate":"1970-01-01T00:00:00.000Z","objectCompound.concept.validEndDate":"2099-12-31T00:00:00.000Z","objectCompound.concept.invalid_reason":null,"precipitantCompound.id":35,"precipitantCompound.name":"green tea leaf","precipitantCompound.unii":"W2ZU1RY8B0","precipitantCompound.inChIKey":null,"precipitantCompound.publicDescription":null,"precipitantCompound.internalComment":null,"precipitantCompound.conceptId":null,"precipitantCompound.enantiomerOfId":null,"precipitantCompound.concept.conceptId":null,"precipitantCompound.concept.conceptName":null,"precipitantCompound.concept.domainId":null,"precipitantCompound.concept.vocabularyId":null,"precipitantCompound.concept.conceptClassId":null,"precipitantCompound.concept.standardConcept":null,"precipitantCompound.concept.conceptCode":null,"precipitantCompound.concept.validStartDate":null,"precipitantCompound.concept.validEndDate":null,"precipitantCompound.concept.invalid_reason":null,"objectMetaboliteCompound.id":null,"objectMetaboliteCompound.name":null,"objectMetaboliteCompound.unii":null,"objectMetaboliteCompound.inChIKey":null,"objectMetaboliteCompound.publicDescription":null,"objectMetaboliteCompound.internalComment":null,"objectMetaboliteCompound.conceptId":null,"objectMetaboliteCompound.enantiomerOfId":null,"objectMetaboliteCompound.concept.conceptId":null,"objectMetaboliteCompound.concept.conceptName":null,"objectMetaboliteCompound.concept.domainId":null,"objectMetaboliteCompound.concept.vocabularyId":null,"objectMetaboliteCompound.concept.conceptClassId":null,"objectMetaboliteCompound.concept.standardConcept":null,"objectMetaboliteCompound.concept.conceptCode":null,"objectMetaboliteCompound.concept.validStartDate":null,"objectMetaboliteCompound.concept.validEndDate":null,"objectMetaboliteCompound.concept.invalid_reason":null,"enzymes.id":null,"enzymes.name":null,"enzymes.conceptId":null,"enzymes.experiment_enzyme_xref.enzymeId":null,"enzymes.experiment_enzyme_xref.experimentId":null,"transporters.id":4,"transporters.name":"MATE2-K (SLC47A2)","transporters.conceptId":null,"transporters.experiment_transporter_xref.experimentId":22,"transporters.experiment_transporter_xref.transporterId":4,"quantifiedMetabolites.id":null,"quantifiedMetabolites.name":null,"quantifiedMetabolites.unii":null,"quantifiedMetabolites.inChIKey":null,"quantifiedMetabolites.publicDescription":null,"quantifiedMetabolites.internalComment":null,"quantifiedMetabolites.conceptId":null,"quantifiedMetabolites.enantiomerOfId":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.com":null,"quantifiedMetabolites.experiment_quantified_metabolite_xref.exp":null,"study.id":10,"study.uid":"NPDI-7LwuzA","study.name":"Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein","study.napdiIdentifier":"PMID:26426900","study.overallSummary":"Green tea catechins inhibit the function of organic anion transporting polypeptides (OATPs) that mediate the uptake of a diverse group of drugs and endogenous compounds into cells. The present study was aimed at investigating the effect of green tea and its most abundant catechin epigallocatechin gallate (EGCG) on the transport activity of several drug transporters expressed in enterocytes, hepatocytes and renal proximal tubular cells such as OATPs, organic cation transporters (OCTs), multidrug and toxin extrusion proteins (MATEs), and P-glycoprotein (P-gp). Uptake of the typical substrates metformin for OCTs and MATEs and bromosulphophthalein (BSP) and atorvastatin for OATPs was measured in the absence and presence of a commercially available green tea and EGCG. Transcellular transport of digoxin, a typical substrate of P-gp, was measured over 4 hours in the absence and presence of green tea or EGCG in Caco-2 cell monolayers. OCT1-, OCT2-, MATE1- and MATE2-K-mediated metformin uptake was significantly reduced in the presence of green tea and EGCG (P &lt; 0.05). BSP net uptake by OATP1B1 and OATP1B3 was inhibited by green tea [IC50 2.6% (v/v) and 0.39% (v/v), respectively]. Green tea also inhibited OATP1B1- and OATP1B3-mediated atorvastatin net uptake with IC50 values of 1.9% (v/v) and 1.0% (v/v), respectively. Basolateral to apical transport of digoxin was significantly decreased in the presence of green tea and EGCG. These findings indicate that green tea and EGCG inhibit multiple drug transporters in vitro. Further studies are necessary to investigate the effects of green tea on prototoypical substrates of these transporters in humans, in particular on substrates of hepatic uptake transporters (e.g. statins) as well as on P-glycoprotein substrates.","study.pubmedId":26426900,"study.embaseId":null,"study.croIdentifier":"na","study.croInformation":"University of Erlangen Nuremberg","study.dateStart":null,"study.dateEnd":null,"study.internalComment":"This is a study reported in the literature. The data was entered by JS and reviewed by JY.","study.status":"published","study.compoundId":null,"study.naturalProductUid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProductSampleId":null,"study.studySourceTypeId":1,"study.naturalProduct.uid":"NP-028bf4b2-0bc6-458f-b86f-6930ce40efc6","study.naturalProduct.binomial":"Camellia sinensis","study.naturalProduct.name":"Green tea","study.naturalProduct.itis":null,"study.naturalProduct.srs":"e9698137-24da-46f8-a70e-43e27691491f","study.naturalProduct.source_id":"","study.naturalProduct.conceptId":null,"study.naturalProduct.concept.conceptId":null,"study.naturalProduct.concept.conceptName":null,"study.naturalProduct.concept.domainId":null,"study.naturalProduct.concept.vocabularyId":null,"study.naturalProduct.concept.conceptClassId":null,"study.naturalProduct.concept.standardConcept":null,"study.naturalProduct.concept.conceptCode":null,"study.naturalProduct.concept.validStartDate":null,"study.naturalProduct.concept.validEndDate":null,"study.naturalProduct.concept.invalid_reason":null,"study.compound.id":null,"study.compound.name":null,"study.compound.unii":null,"study.compound.inChIKey":null,"study.compound.publicDescription":null,"study.compound.internalComment":null,"study.compound.conceptId":null,"study.compound.enantiomerOfId":null,"study.studySourceType.id":1,"study.studySourceType.name":"Published report","testSystem.id":22,"testSystem.name":"HEK293 transfected cells","testSystem.sortOrder":0,"testSystem.conceptId":null,"testSystem.categoryId":4,"testSystem.category.id":4,"testSystem.category.name":"Transfected / siRNA knock-out / injected cells","testSystem.category.sortOrder":4,"testSystem.category.requiresCytochromeB5":false,"cytochromeB5.id":null,"cytochromeB5.name":null,"cytochromeB5.sortOrder":null,"cytochromeB5.conceptId":null,"controlDataExperiment.id":74,"controlDataExperiment.uid":"NPDI-WsMb-g","controlDataExperiment.name":"Metformin uptake in MATE2-K cells","controlDataExperiment.overallEffect":1,"controlDataExperiment.isControlData":true,"controlDataExperiment.isIc50Shift":false,"controlDataExperiment.croCutoff":"NA","controlDataExperiment.croIdentifier":null,"controlDataExperiment.comment":null,"controlDataExperiment.experimentalConditionsComment":null,"controlDataExperiment.resultsComment":null,"controlDataExperiment.internalComment":null,"controlDataExperiment.objectCompoundId":49,"controlDataExperiment.objectMetaboliteCompoundId":null,"controlDataExperiment.precipitantCompoundId":null,"controlDataExperiment.cytochromeB5Id":null,"controlDataExperiment.studyId":10,"controlDataExperiment.experimentTypeId":7,"controlDataExperiment.testSystemId":22,"controlDataExperiment.ic50ShiftExperimentId":null,"controlDataExperiment.controlDataExperimentId":null,"controlDataExperiment.controlDataForExperimentId":null,"controlDataExperiment.naturalProductSampleId":null,"controlDataExperiment.experimentType.id":7,"controlDataExperiment.experimentType.name":"In Vitro Transporter Kinetics","controlDataExperiment.experimentType.isInVitro":true,"controlDataExperiment.experimentType.isTransporter":true,"controlDataExperiment.experimentType.isEnzyme":false,"controlDataExperiment.experimentType.purl":"http://purl.obolibrary.org/obo/DIDEO_00005003","controlDataExperiment.objectCompound.id":49,"controlDataExperiment.objectCompound.name":"metformin","controlDataExperiment.objectCompound.unii":null,"controlDataExperiment.objectCompound.inChIKey":"XZWYZXLIPXDOLR-UHFFFAOYSA-N","controlDataExperiment.objectCompound.publicDescription":null,"controlDataExperiment.objectCompound.internalComment":null,"controlDataExperiment.objectCompound.conceptId":1503297,"controlDataExperiment.objectCompound.enantiomerOfId":null,"controlDataExperiment.precipitantCompound.id":null,"controlDataExperiment.precipitantCompound.name":null,"controlDataExperiment.precipitantCompound.unii":null,"controlDataExperiment.precipitantCompound.inChIKey":null,"controlDataExperiment.precipitantCompound.publicDescription":null,"controlDataExperiment.precipitantCompound.internalComment":null,"controlDataExperiment.precipitantCompound.conceptId":null,"controlDataExperiment.precipitantCompound.enantiomerOfId":null,"controlDataExperiment.objectMetaboliteCompound.id":null,"controlDataExperiment.objectMetaboliteCompound.name":null,"controlDataExperiment.objectMetaboliteCompound.unii":null,"controlDataExperiment.objectMetaboliteCompound.inChIKey":null,"controlDataExperiment.objectMetaboliteCompound.publicDescriptio":null,"controlDataExperiment.objectMetaboliteCompound.internalComment":null,"controlDataExperiment.objectMetaboliteCompound.conceptId":null,"controlDataExperiment.objectMetaboliteCompound.enantiomerOfId":null,"controlDataExperiment.enzymes.id":null,"controlDataExperiment.enzymes.name":null,"controlDataExperiment.enzymes.conceptId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.enzymeId":null,"controlDataExperiment.enzymes.experiment_enzyme_xref.experiment":null,"controlDataExperiment.transporters.id":4,"controlDataExperiment.transporters.name":"MATE2-K (SLC47A2)","controlDataExperiment.transporters.conceptId":null,"controlDataExperiment.transporters.experiment_transporter_xref.":4,"controlDataExperiment.quantifiedMetabolites.id":null,"controlDataExperiment.quantifiedMetabolites.name":null,"controlDataExperiment.quantifiedMetabolites.unii":null,"controlDataExperiment.quantifiedMetabolites.inChIKey":null,"controlDataExperiment.quantifiedMetabolites.publicDescription":null,"controlDataExperiment.quantifiedMetabolites.internalComment":null,"controlDataExperiment.quantifiedMetabolites.conceptId":null,"controlDataExperiment.quantifiedMetabolites.enantiomerOfId":null,"controlDataExperiment.quantifiedMetabolites.experiment_quantifi":null,"experimentAnswers.id":2174,"experimentAnswers.text":"8","experimentAnswers.answerId":null,"experimentAnswers.experimentId":22,"experimentAnswers.questionId":41,"experimentAnswers.question.id":41,"experimentAnswers.question.text":"Incubation pH","experimentAnswers.question.required":false,"experimentAnswers.question.maxAnswers":null,"experimentAnswers.question.type":"STRING","experimentAnswers.question.conceptId":null,"experimentAnswers.question.answers.id":null,"experimentAnswers.question.answers.text":null,"experimentAnswers.question.answers.sortOrder":null,"experimentAnswers.question.answers.conceptId":null,"experimentAnswers.question.answers.questionId":null,"experimentAnswers.answer.id":null,"experimentAnswers.answer.text":null,"experimentAnswers.answer.sortOrder":null,"experimentAnswers.answer.conceptId":null,"experimentAnswers.answer.questionId":null}]