In Vitro Enzyme Induction Experiment

Induction was detected.  Cutoff used — Not specified


  • CYP1A2 4312402

Cell system HepG2 cell line

Figure 3


the protein expression of CYP1A2 showed an increased in relative values from 0.5uM onwards as compared to control. Concentration-dependent effects was only observed from 0.1 to 1.0 uM. The mean relative intensity at 1 and 10 lM showed an increase in value, which corresponded with the positive control (omepra- zole). The effect of mitragynine on the CYP1A2 protein expression was consistent with the effect observed on CYP1A2 mRNA expression, although the concentration-dependent effects were not as obvious.

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Protein expression

0.1, 0.5, 1, 5, 10, 25 uM

48 hours

Control Experiment