In Vitro Enzyme Inhibition Experiment

Inhibition of UGT by (-)-Epicatechin (Screening)

Negligible Inhibition was detected.  Cutoff used — 50% inhibition vs. control



4-methylumbelliferone glucuronide

  • UGT

Cell fraction Pooled human intestinal microsomes -7999663


(-)-Epicatechin did not significantly inhibit UGT enzymes--observed UGT activity was 110% of control.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Purified green tea catechins were tested as inhibitors of UGT enzymes as described in Gufford et al., 2014 (Drug Metab Dispos. 2014 Oct;42(10):1675-83); information regarding incubation volume, time, and protien linearity were obtain therein.
Bovine serum albumin (0.05%) was added into the incubation system.

0.2 mg/mL

Commercially available


150 µL

20 min



Not available

100 µM

100 µM

Control Experiment