Efflux of Berberine through P-gp CSV JSON

In Vitro Transporter Kinetics Experiment

Efflux of Berberine through P-gp

No Transport Activity was detected.  Cutoff used —

Papp >1.5x10-6 cm·s-1 is considered to be highly penetrative; therefore, the Papp of the four alkaloids assessed in the present

berberine 19012197

  • P-gp (ABCB1)

Cell system Caco-2 cells

Results

Above results for 10 μmol

Papp A-B
20 μmol: 3.53
30 μmol: 2.85
60 μmol: 2.98
100 μmol: 2.68

Papp B-A
20 μmol: 31.30
30 μmol: 20.81
60 μmol: 25.30
100 μmol: 25.90

Ratio PappB-A / PappA-B
20 μmol: 8.87
30 μmol: 7.30
60 μmol: 8.49
100 μmol: 9.66

Table IV

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

5x10^4/cm^2

12-well plate

Transwell

21

Assay Conditions

60 min

37 degrees C

7.4

10, 20, 30, 60, 100 μmol*mL^(-1)