P-gp mediated digoxin transport in Caco-2 cells CSV JSON

In Vitro Transporter Kinetics Experiment

P-gp mediated digoxin transport in Caco-2 cells

Transport Activity was detected.  Cutoff used — NA

digoxin 1326303

  • P-gp (ABCB1)

Cell system Caco-2 cells

Results

Digoxin transport in Caco-2 cells was greater in the B-A direction compared to in the A-B direction (3.2%/h vs. 0.74%/h). In the presence of the P-go inhibitor zosquidar (1 µM), net efflux of digoxin was completely inhibited.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

transwell plates

7

Viability and Function

> 200 Ω*cm2

Assay Conditions

4 h

37 deg C

5 µM