In Vitro Enzyme Inhibition Experiment

Inhibition of UGT by (-)-Epigallocatechin (Screening)

Negligible Inhibition was detected.  Cutoff used — 50% inhibition vs. control

4-methylumbelliferone

epigallocatechin

4-methylumbelliferone glucuronide

  • UGT

Cell fraction Pooled human intestinal microsomes -7999663

The precipitant used was (-)-epigallocatechin.

Results

(-)-Epigallocatechin did not significantly inhibit UGT enzymes--observed UGT activity was 110% of control.

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Purified green tea catechins were tested as inhibitors of UGT enzymes as described in Gufford et al., 2014 (Drug Metab Dispos. 2014 Oct;42(10):1675-83); information regarding incubation volume, time, and protien linearity were obtain therein.
Bovine serum albumin (0.05%) was added into the incubation system.

0.2 mg/mL

Commercially available

1410074

150 µL

20 min

UDPGA

Available

Not available

100 µM

100 µM

Control Experiment