- Natural product: Goldenseal (Hydrastis canadensis)
- Associated study: Enzyme-inducing effects of berberine on cytochrome P450 1A2 in vitro and in vivo
In Vitro Enzyme Induction Experiment
Induction of CYP1A2 Measured by mRNA Expression
Induction was detected. Cutoff used — not specified
acetaminophen 1125315
- CYP1A2 4312402
Cell system HepG2 cell line
Results
CYP1A2 mRNA expression in HepG2 cells treated with BER exhibited an induction effect, which significantly increased by 1.47, 5.90, 11.71, and 16.11-fold exposed to BER at 0.5, 1.5, 3.0, and 4.5 μg/ml, respectively (Fig. 2) SD estimated from Fig 2
Values changed from a fold change to a percent for repo
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
mRNA expression
200,000 cells/mL
76 h
Total RNA was extracted and reverse transcripts
48 h
BCA protein assay
72 h
Phenacetin O-deethylation