Induction of CYP1A2 Measured by mRNA Expression CSV JSON

In Vitro Enzyme Induction Experiment

Induction of CYP1A2 Measured by mRNA Expression

Induction was detected.  Cutoff used — not specified

acetaminophen 1125315

  • CYP1A2 4312402

Cell system HepG2 cell line

Results

CYP1A2 mRNA expression in HepG2 cells treated with BER exhibited an induction effect, which significantly increased by 1.47, 5.90, 11.71, and 16.11-fold exposed to BER at 0.5, 1.5, 3.0, and 4.5 μg/ml,  respectively (Fig. 2) SD estimated from Fig 2

Values changed from a fold change to a percent for repo

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

mRNA expression

200,000 cells/mL

76 h

Total RNA was extracted and reverse transcripts

48 h

BCA protein assay

72 h

Phenacetin O-deethylation