Cat's Claw 1/1000 Extracts on CYP3A4 mRNA Expression CSV JSON

In Vitro Nuclear Receptor Induction Experiment

Cat's Claw 1/1000 Extracts on CYP3A4 mRNA Expression

Induction was detected.  Cutoff used —

Not available

cat's claw extract

  • CYP3A4 4306811

Cell system PXR-transfected hepatic cells

Results

CYP3A4 mRNA level (% Rifampicin): 175 ± 2 (mean ± SEM)
p-value < 0.0001

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

For the analysis of CYP3A4 expression, ~500,000 DPX2 cells were seeded on a 12-well plate and cultured for 24 h. Cells were treated with herbal extracts or chemicals for another 24 h and then washed with PBS. Total RNA of the cells was extracted using TRIzolTM Reagent and 2 µg of them was used for reverse transcription. Quantitative RTPCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems by Thermo Fisher Scientific, Warrington, UK). mRNA levels of CYP3A4 were normalized to Gapdh.

mRNA expression

~500,000 DPX2 cells seeded on a 12-well plate