In Vitro Enzyme Inhibition Experiment
Inhibition of UGT by Nicardipine in HIM (Screening)
Inhibition was detected. Cutoff used — 50% inhibition vs. control
Cell fraction Individual human intestinal microsomes -7999704
Nicardipine was used as the positive control inhibitor.
Percent inhibition was estimated from Fig. S1.
|Sample||Compound measured||Value||Measurement||Study sequence||Additional information||N replicates|
Purified green tea catechins were tested as inhibitors of UGT enzymes as described in Gufford et al., 2014 (Drug Metab Dispos. 2014 Oct;42(10):1675-83); information regarding incubation volume, time, and protien linearity were obtain therein.
Bovine serum albumin (0.05%) was added into the incubation system.