- Natural product: Green tea (Camellia sinensis)
- Associated study: Identification of Intestinal UDP-Glucuronosyltransferase Inhibitors in Green Tea (Camellia sinensis) Using a Biochemometric Approach: Application to Raloxifene as a Test Drug via In Vitro to In Vivo Extrapolation
In Vitro Enzyme Inhibition Experiment
Inhibition of UGT by Nicardipine in HIM (Screening)
Inhibition was detected. Cutoff used — 50% inhibition vs. control
- UGT
Cell fraction Individual human intestinal microsomes -7999704
Nicardipine was used as the positive control inhibitor.
Results
Percent inhibition was estimated from Fig. S1.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Purified green tea catechins were tested as inhibitors of UGT enzymes as described in Gufford et al., 2014 (Drug Metab Dispos. 2014 Oct;42(10):1675-83); information regarding incubation volume, time, and protien linearity were obtain therein.
Bovine serum albumin (0.05%) was added into the incubation system.
0.2 mg/mL
Commercially available
1410074
150 µL
20 min
UDPGA
Available
Not available
100 µM
400 µM