- Natural product: Licorice (Glycyrrhiza glabra)
- Associated study: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice)
Metabolomics Study
Metabolomic Profiling of Gancao Phytoconstituents
Results
Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
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PCA Plot
Instructions:
Select the number of dimensions to view in the PCA plot.
Each dimension corresponds with a principal component selected.
Data points represent the following samples (click to show/hide):
Click any dot in the plot to get more information about that particular data
point in the table below the plot.
In the 3-D plot, you can double click
anywhere in the space to focus the camera on that particular point and
scroll to zoom in and out
Principal component analysis (PCA) scores plot
Squares represent samples linked to pharmacology and/or chemical characterization experiments.
Experimental Conditions
Ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) (Waters Corporation, Milford, MA, USA) were used for the analysis of chemical components in Gancao extract. A 100 mm 2.1 mm UPLC BEH C-18 column (Acquity 1.7 mm) together with a gradient of aqueous acetonitrile with 0.1% of formic acid were used for chemical separation. QTOFMS was conducted in a negative mode with electrospray ionization. The data were collected by MassLynx 4.1 (Waters Corporation, Milford, MS, USA) and further analyzed by SIMCA-P (Umetrics, Kinnelon, NJ, USA).
Ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry
Electrospray ionization
Negative
Gradient of aqueous acetonitrile with 0.1% of formic acid
100 mm 2.1 mm UPLC BEH C-18 column (Acquity 1.7 mm)