Ivy leaf (Hedera helix)
Ivy leaf mixed parts extract Nuclear Receptor Induction study - Ole Miss CSV JSON

PXR is a key regulator for the expression of CYP 3A4 which is involved in the metabolism of majority of clinical drugs. Induction of PXR has been known to be associated with pharmacokinetic drug interactions.
AhR is involved in regulation of the expression of certain CYP isoforms. Phytochemicals could be the AhR agonists and induction of AhR could play a role in herb-drug interation. 

Ivy leaf (Hedera helix)

mixed parts

36017806

1 . Ivy leaf - In Vitro Nuclear Receptor Induction AHR Experiment - 1 (id=NPDI-eyxoVQ)

In Vitro Nuclear Receptor Induction Experiment

Induction was detected.  Cutoff used —

An activation of 2-folds or higher. 

ivy leaf extract

  • Aryl hydrocarbon receptor (AHR)

Cell system AhR reporter cells

Results

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Luciferase activity (Reporter gene assay)

200 µL of the proprietary cells / well provided with the AhR assay kit (Indigo Bioscience)

No

30 µg/mL

24 hrs for AhR inducing activity

2 . Ivy leaf - In Vitro Nuclear Receptor Induction AHR Experiment - 2 (id=NPDI-ic0FMw)

In Vitro Nuclear Receptor Induction Experiment

Non-induction was detected.  Cutoff used —

An activation of 2-folds or higher. 

ivy leaf extract

  • Aryl hydrocarbon receptor (AHR)

Cell system AhR reporter cells

Results

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Luciferase activity (Reporter gene assay)

200 µL of the proprietary cells / well provided with the AhR assay kit (Indigo Bioscience)

No

10 µg/mL

24 hrs for AhR inducing activity

3 . Ivy leaf - In Vitro Nuclear Receptor Induction AHR Experiment - 3 (id=NPDI-X5acvg)

In Vitro Nuclear Receptor Induction Experiment

Non-induction was detected.  Cutoff used —

An activation of 2-folds or higher. 

ivy leaf extract

  • Aryl hydrocarbon receptor (AHR)

Cell system AhR reporter cells

Results

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Luciferase activity (Reporter gene assay)

200 µL of the proprietary cells / well provided with the AhR assay kit (Indigo Bioscience)

No

3.3 µg/mL

24 hrs for AhR inducing activity