Indian barberry (Berberis aristata)
Barberry stem extract Nuclear Receptor Induction study - Ole Miss
CSV
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PXR is a key regulator for the expression of CYP 3A4 which is involved in the metabolism of majority of clinical drugs. Induction of PXR has been known to be associated with pharmacokinetic drug interactions.
AhR is involved in regulation of the expression of certain CYP isoforms. Phytochemicals could be the AhR agonists and induction of AhR could play a role in herb-drug interation.
1 . Barberry - In Vitro Nuclear Receptor Induction AHR Experiment - 1 (id=NPDI-kWVgAg)
In Vitro Nuclear Receptor Induction Experiment
Induction was detected. Cutoff used —
An activation of 2-folds or higher.
- Aryl hydrocarbon receptor (AHR)
Cell system AhR reporter cells
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Luciferase activity (Reporter gene assay)
200 µL of the proprietary cells / well provided with the AhR assay kit (Indigo Bioscience)
No
30 µg/mL
24 hrs for AhR inducing activity
2 . Barberry - In Vitro Nuclear Receptor Induction AHR Experiment - 2 (id=NPDI-PnoJBg)
In Vitro Nuclear Receptor Induction Experiment
Induction was detected. Cutoff used —
An activation of 2-folds or higher.
- Aryl hydrocarbon receptor (AHR)
Cell system AhR reporter cells
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Luciferase activity (Reporter gene assay)
200 µL of the proprietary cells / well provided with the AhR assay kit (Indigo Bioscience)
No
10 µg/mL
24 hrs for AhR inducing activity
3 . Barberry - In Vitro Nuclear Receptor Induction AHR Experiment - 3 (id=NPDI-HV4oZQ)
In Vitro Nuclear Receptor Induction Experiment
Induction was detected. Cutoff used —
An activation of 2-folds or higher.
- Aryl hydrocarbon receptor (AHR)
Cell system AhR reporter cells
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Luciferase activity (Reporter gene assay)
200 µL of the proprietary cells / well provided with the AhR assay kit (Indigo Bioscience)
No
3.3 µg/mL
24 hrs for AhR inducing activity