- Natural product: Cat's-claw (Uncaria tomentosa)
- Associated study: Identification of PXR activators from Uncaria tomentosa (Cat’s Claw)
Characterization of Material Study
Metabolomic Profiling of Cat's Claw Extracts
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
The chemical profiling of Cat's claw extracts was conducted using ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS, Waters, Milford, MA). An UPLC BEH C-18 column was utilized for chemical separation. The mobile phase was aqueous acetonitrile containing 0.1% formic acid and was delivered in a gradient elution from 2% to 98% acetonitrile. QTOFMS was performed in a positive mode. 1'-OH-MDZ was also analyzed by UPLC-QTOFMS using the same column. The UPLC-QTOFMS data for herbal extracts were collected using MassLynx 4.1 (Waters Corp., Milford, MA) and further analyzed by orthogonal partial least-squares discriminant analysis using SIMCA-P (Umetrics, Kinnelon, NJ).
quadrupole time-of-flight mass spectrometry
Positive
ultra-performance liquid chromatography
UPLC BEH C-18