Inhibition of OATP1B3 by (-)-epigallocatechin gallate CSV JSON

Inhibition of OATP1B3 by (-)-epigallocatechin gallate

Object compound

Precipitant

Transporters involved

Test system used

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Cell Culture Conditions

Density

7.0 x 10^5 cells/well

Plate type

12-well poly-D-lysine coated plates

Days after plating

48 h

Assay Conditions

Incubation time

5 min

Incubation temperature

37 deg C

Object concentrations tested

1 µM

Precipitant concentrations tested

100 µM (percent inhibition); 0-200 µM (IC50 determination)

Precipitant source

Prof. Toshiyuki Kan (Department of Synthetic Organic and Medicinal Chemistry, University of Shizuoka, Japan)

NOTE