In Vitro Transporter Kinetics Experiment

Mitragynine P-gp transport activity

No Transport Activity was detected.  Cutoff used — ER of 2

mitragynine

  • P-gp (ABCB1)

Cell system Caco-2 cells

Results

Results above for 5 uM
ER for 10 uM values reported: 1.1 N=3
(Table 3)

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

70 000 cells/well

200 μL

Transwell

2

30–42

Viability and Function

>400 ohm/cm2

TEER measurements and Ly permeability.

Assay Conditions

120 min

37 deg C

7.4

5,10 uM