Berberine Transport by OCT2 CSV JSON

In Vitro Transporter Kinetics Experiment

Berberine Transport by OCT2

Transport Activity was detected.  Cutoff used — not specified

Berberine 19012197

  • OCT2 (SLC22A2)

Transfected / siRNA knock-out / injected cells MDCK transfected cells

Results

Fig 5 estimate of uptake at 10 min (accumulation)

Fig. 6. Concentration-dependent uptake of berberine mediated by hOCT2 or hOCT3. Up- take was carried out in parallel in mock, hOCT2- and hOCT3-transfected MDCK cells. Up- take in mock cells was subtracted from hOCT2 or hOCT3 cells to obtain transporter- specific uptake. The uptake was performed within 2 min. Data are expressed as mean ± SD (n = 3).

Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

24 well

2-3

Assay Conditions

2 min

37 deg C

0.25-25 μM