Inhibition of P-gp by (-)epigallocatecin gallate CSV JSON

In Vitro Transporter Inhibition Experiment

Inhibition of P-gp by (-)epigallocatecin gallate

Inhibition was detected.  Cutoff used — NA

digoxin 1326303

  • P-gp (ABCB1)

Cell system Caco-2 cells

Results

In the presence of (-)-epigallocatechin gallate, the B-A transport of digoxin significantly decreased from 3.2%/h to 1.6%/h (P < 0.01 to 0.001). In the presence of (-)-epigallocatechin gallate, the B-A transport was greater than the A-B transport.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

transwell plates

7

Viability and Function

>200 Ω

Assay Conditions

4 h

37 deg C

5 µM

1 µM

Prof. Toshiyuki Kan (Department of Synthetic Organic and Medicinal Chemistry, University of Shizuoka, Japan)

Control Experiment