- Natural product: Kratom (Mitragyna speciosa)
- Associated study: Evaluation of selected Malaysian medicinal plants on phase I drug metabolizing enzymes, CYP2C9, CYP2D6 and CYP3A4 activities in vitro
In Vitro Enzyme Inhibition Experiment
POS. CONTROL: Inhibition of CYP3A4 by ketoconazole
Inhibition was detected. Cutoff used — Not specified.
- CYP3A4 4306811
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Not available
Positive control
Results
Positive control
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Positive controls sulfaphenazole, quinidine, and ketoconazole were purchased from Sigma Chemicals (St. Louis, USA). This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.
The minus-P450 wells (using luciferin-free water) were negative controls.
Commercially available
100 µL
50 min
MgCl2
NADPH regenerating system
Not available
Not available
0.02-200 µM