Evaluation of Time-Dependent CES1 Inhibition by THC (30 min) CSV JSON

Evaluation of Time-Dependent CES1 Inhibition by THC (30 min)

Object compound

Precipitant

Object metabolite investigated

Enzymes involved in metabolite pathway

Test system used

Cell fraction Human kidney S9 fraction

IC₅₀ pre-incubation 0 min (A) = 3.91 µM
IC₅₀ pre-incubation 30 min (A) = 11.2 µM
B/A = 2.85

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Protein concentration

20 µg/mL

Test system preparation

Commercially available

Incubation volume

100 µL

Incubation time

15 min

Object concentrations tested

1 mM

Precipitant pre-incubation volume

30 min

Precipitant pre-incubation time

30 min

Precipitant concentrations tested

0 - 50 µg/mL

NOTE