Inhibition of CYP2B6 by G. glabra Licorice extract CSV JSON

Natural product: Licorice (Glycyrrhiza glabra)
Associated study: Cytochrome P450 inhibition by licorice species Glycyrrhiza glabra L. and fourteen licorice constituents

In Vitro Enzyme Inhibition Experiment

Inhibition of CYP2B6 by G. glabra Licorice extract

Inhibition was detected. Cutoff used —
> 100 µg/mL

Object compound

bupropion 750982

Precipitant

ethanolic extract of licorice g. glabra

Object metabolite investigated

hydroxybupropion 4037950

Enzymes involved in metabolite pathway

CYP2B6 4308333

Test system used

Cell fraction Pooled human liver microsomes -7999662

Results

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Experimental Conditions

cocktail assay, IC50 values were determined according to the method of Li et al. (2015).

Li, G., Huang, K., Nikolic, D., van Breemen, R.B., 2015. High-throughput cytochrome P450 cocktail inhibition assay for assessing drug-drug and drug-botanical interactions. Drug Metab. Dispos. 43, 1670–1678.

Cell density

0.2 mg/mL

Incubation time

10 min

Co-factors

NADPH

Object concentrations tested

12 µM

Precipitant concentrations tested

11 concentrations from 0.005–250 μg/mL

NOTE

Inhibition of CYP2B6 by G. glabra Licorice extract CSV JSON

In Vitro Enzyme Inhibition Experiment

Inhibition was detected. Cutoff used — > 100 µg/mL

Results

Experimental Conditions

Inhibition was detected. Cutoff used —
> 100 µg/mL