- Natural product: Cannabis (Cannabis sativa)
- Associated study: Characterization of the structural determinants required for potent mechanism-based inhibition of human cytochrome P450 1A1 by cannabidiol
In Vitro Enzyme Inhibition Experiment
Cannbidiol-2'-monomethyl ether
Inhibition was detected. Cutoff used — Not specified
- CYP1A1 4173297
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Not available
Results
%inhibition estimated from Figure 2.
IC50pre-incubation at 0 min (Table 1).
IC50pre-incubation at 20 min - 1.90
Kinact/KI converted from /min/mmol to /min/μM (table 2).
All other values from Table 2.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Commercially available
200 µL
30 min
NADPH
20 µL
180 µL
5 min
NADPH with precipitant