- Natural product: Cannabis (Cannabis sativa)
- Associated study: Comparison in the in vitro inhibitory effects of major phytocannabinoids and polycyclic aromatic hydrocarbons contained in marijuana smoke on cytochrome P450 2C9 activity.
In Vitro Enzyme Inhibition Experiment
rCYP2C9: CBN-diclofenac
Inhibition was detected. Cutoff used — Not specified
- CYP2C9 4309227
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 No
Results
Tables 2 and 3
Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
---|
Experimental Conditions
2 pmol
Commercially available
250 μL
20 min
MgCl2
NADPH regenerating system
5 min
NADPH with precipitant