Enzyme kinetics of CYP3A4 CSV JSON

Enzyme kinetics of CYP3A4

Object compound

Object metabolite investigated

Enzymes involved in metabolite pathway

Test system used

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed

Km and Vmax from Table 2

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.

Method for determination of overall effect

Metabolite formation study

Test system preparation

Commercially available

Incubation time

50 min

Co-factors

NADPH regenerating system

Protein linearity

Not available

Time linearity

Available

Object concentrations tested

0–100µM

NOTE