- Natural product: Goldenseal (Hydrastis canadensis)
- Associated study: R21 Goldenseal Project
In Vitro Transporter Inhibition Experiment
Inhibition of THTR2 by Berberine
Inhibition was detected. Cutoff used —
Inhibition
- THTR2 (SLC19A3)
Transfected / siRNA knock-out / injected cells HEK293 transfected cells
Berberine is a constituent of Goldenseal.
Results
Goldenseal extract, normalized to berberine content, was the strongest inhibitor of OCT3, PMAT, and THTR2 transporter activity.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
DMSO was used as vehicle.
1×10^5 cells/well
50 µL of precipitant per well, ~ 1 mg per well of protein content (not used in calculations)
96 well
16-24h
p19
N/A
N/A
3 minutes
37°C
7.4
0.03 µM
0, 0.27, 0.82, 2.47, 7.41, 22.22, 66.67, 200 µM