Experiment #NPDI-_5Ix4Q CSV JSON

In Vitro Enzyme Inhibition Experiment

Inhibition was detected.  Cutoff used — N/A

4-nitrophenol

epigallocatechin gallate 19058494

4-nitrocatechol

  • CYP2E1 4173608

Recombinant expression system Not available
Cytochrome B5 Not available

Results

The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.

Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.

CYP: 20 pmol/mL

In-house preparation

0.5 mL

30 min at pH 6.8

NADPH regenerating system

Not available

Not available

100 µM

50 µM