Inhibition of PMAT by Goldenseal CSV JSON

In Vitro Transporter Inhibition Experiment

Inhibition of PMAT by Goldenseal

Inhibition was detected.  Cutoff used —

Inhibition

mpp+

  • ENT4 (SLC29A4)

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Goldenseal was normalized to berberine content.

Results

Goldenseal extract, normalized to berberine content, was the strongest inhibitor of OCT3, PMAT, and THTR2 transporter activity.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

DMSO was used as vehicle.

Cell Culture Conditions

1×10^5 cells/well

50 µL of precipitant per well, ~ 1 mg per well of protein content (not used in calculations)

96 well

16-24h

p17

Viability and Function

N/A

N/A

Assay Conditions

2 minutes

37°C

6.6

10 µM

0, 0.02, 0.07, 0.22, 0.65, 1.94, 5.83, 17.5 µM

Dr. Nicholas Oberlies (UNCG)