Inhibition of PMAT by Goldenseal CSV JSON

Natural product: Goldenseal (Hydrastis canadensis)
Associated study: R21 Goldenseal Project

In Vitro Transporter Inhibition Experiment

Inhibition of PMAT by Goldenseal

Inhibition was detected. Cutoff used —
Inhibition

Object compound

mpp+

Precipitant

goldenseal root extract

Transporters involved

ENT4 (SLC29A4)

Test system used

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Goldenseal was normalized to berberine content.

Results

Goldenseal extract, normalized to berberine content, was the strongest inhibitor of OCT3, PMAT, and THTR2 transporter activity.

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Experimental Conditions

DMSO was used as vehicle.

Cell Culture Conditions

Density

1×10^5 cells/well

Protein amount/well or concentration

50 µL of precipitant per well, ~ 1 mg per well of protein content (not used in calculations)

Plate type

96 well

Days after plating

16-24h

Passage number

p17

Viability and Function

TEER

N/A

Barrier integrity method

N/A

Assay Conditions

Incubation time

2 minutes

Incubation temperature

37°C

Incubation pH

6.6

Object concentrations tested

10 µM

Precipitant concentrations tested

0, 0.02, 0.07, 0.22, 0.65, 1.94, 5.83, 17.5 µM

Precipitant source

Dr. Nicholas Oberlies (UNCG)

NOTE

Inhibition of PMAT by Goldenseal CSV JSON

In Vitro Transporter Inhibition Experiment

Inhibition was detected. Cutoff used — Inhibition

Results

Experimental Conditions

Inhibition was detected. Cutoff used —
Inhibition