- Natural product: Green tea (Camellia sinensis)
- Associated study: Identification of Intestinal UDP-Glucuronosyltransferase Inhibitors in Green Tea (Camellia sinensis) Using a Biochemometric Approach: Application to Raloxifene as a Test Drug via In Vitro to In Vivo Extrapolation
In Vitro Enzyme Inhibition Experiment
Inhibition of UGT by (-)-Epicatechin (Screening)
Negligible Inhibition was detected. Cutoff used — 50% inhibition vs. control
Cell fraction Pooled human intestinal microsomes -7999663
(-)-Epicatechin did not significantly inhibit UGT enzymes--observed UGT activity was 110% of control.
|Sample||Compound measured||Value||Measurement||Study sequence||Additional information||N replicates|
Purified green tea catechins were tested as inhibitors of UGT enzymes as described in Gufford et al., 2014 (Drug Metab Dispos. 2014 Oct;42(10):1675-83); information regarding incubation volume, time, and protien linearity were obtain therein.
Bovine serum albumin (0.05%) was added into the incubation system.