Zidovudine Glucoridination Kinetics in Rat Liver Microsomes Without BSA CSV JSON

Zidovudine Glucoridination Kinetics in Rat Liver Microsomes Without BSA

Object compound

Object metabolite investigated

Enzymes involved in metabolite pathway

Test system used

Cell fraction Rat liver microsomes

Table 4 - RLM Without 1% BSA

Compound measured	Value	Measurement	Additional information	N replicates
zidovudine glucuronide	0.09µL/mg protein/min	CL_{int total} Mean		3
zidovudine glucuronide	8.84 ± 1.19mM	K_m Mean ± SD	P-value: P < 0.05	3
zidovudine glucuronide	770 ± 40pmol/mg protein/min	V_max Mean ± SD		3

Without 1% bovine serum albumin (BSA)

Method for determination of overall effect

Metabolite formation study

Protein concentration

6 mg/mL

Test system preparation

In-house preparation

Incubation time

90 min

Protein linearity

Available

Time linearity

Available

Object concentrations tested

0 - 6 mM

NOTE