Inhibition of PMAT by Berberine CSV JSON

In Vitro Transporter Inhibition Experiment

Inhibition of PMAT by Berberine

Inhibition was detected.  Cutoff used —

Inhibition

mpp+

berberine 19012197

  • ENT4 (SLC29A4)

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Berberine is a constituent of Goldenseal. 

Results

Goldenseal extract, normalized to berberine content, was the strongest inhibitor of OCT3, PMAT, and THTR2 transporter activity.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

DMSO was used as vehicle.

Cell Culture Conditions

1×10^5 cells/well

50 µL of precipitant per well, ~ 1 mg per well of protein content (not used in calculations)

96 well

16-24h

P17

Viability and Function

N/A

N/A

Assay Conditions

2 minutes

37°C

6.6

10 µM

0, 0.27, 0.82, 2.47, 7.41, 22.22, 66.67, 200 µM