- Natural product: Cannabis (Cannabis sativa)
- Associated study: Comparison in the in vitro inhibitory effects of major phytocannabinoids and polycyclic aromatic hydrocarbons contained in marijuana smoke on cytochrome P450 2C9 activity.
In Vitro Enzyme Inhibition Experiment
pHLM: THC-warfarin
Inhibition was detected. Cutoff used — Not specified
- CYP2C9 4309227
Cell fraction Pooled human liver microsomes -7999662
Results
Tables 2, 3, and 4.
IC50 pre-incubation recorded at 20 min
Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
---|
Experimental Conditions
20 μg
Commercially available
200 μL
20 min
MgCl2
NADPH regenerating system
5 min
NADPH with precipitant