- Natural product: Goldenseal (Hydrastis canadensis)
- Associated study: Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1)
In Vitro Transporter Kinetics Experiment
P-gp Transport of Berberine
Transport Activity was detected. Cutoff used — not specified
berberine 19012197
- P-gp (ABCB1)
Transfected / siRNA knock-out / injected cells MDCK transfected cells
Results
Fig 6b estimate at 60 min
Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
---|
Experimental Conditions
1 x 10^8 cm^2
12 well
Cellstar or Thincert
2-4
60 min
37 deg C
7.4
25 μM