P-gp Permeability of Berberine Induced by Verapamil CSV JSON

In Vitro Transporter Inhibition Experiment

P-gp Permeability of Berberine Induced by Verapamil

Inhibition was detected.  Cutoff used — not specified

berberine 19012197

verapamil

  • P-gp (ABCB1)

Cell system Caco-2 cells

Results

AP-BL/BL-AP

Berberine 7.30±0.02 

BER with verapamil 2.00±0.20 (p<0.01)
Table V

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

1 x 10^6 cells/mL

12 well

Transwell

21

Assay Conditions

30 min

37 deg C

7.4

30 μmol x l^(-1)

100 μmol x l^(-1)

Sigma-Aldrich (St. Louis, MO, USA)