EGCG inhibition of UGT1A1 CSV JSON

In Vitro Enzyme Inhibition Experiment

EGCG inhibition of UGT1A1

Inhibition was detected.  Cutoff used — > 20% inhibition at the highest tested concentrations

17-beta-estradiol

epigallocatechin gallate 19058494

17-beta-estradiol-3-O-glucuronide

  • UGT1A1 40782950

Cell fraction Pooled human liver microsomes -7999662

Concentrations of herbal extracts in screening incubations represent the recommended daily intake (RDI) of each extract dissolved in 53 L, 5.3 L, and 0.53 L. For EGCG, inhibition at the represented RDI was compared with the recommended daily dose (250 mg) of EGCG. The value per dose index (VDI) value was 32.1 L, which is the highest value of all extracts tested, indicating the highest potential for inhibition.

Results

Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Precipitant concentrations were estimated from Fig. 2.

0.5 mg/mL

Commercially available

250 µL

30 min

UDPGA

Not available

Not available

25 µM

0.05-500 µg/mL