- Natural product: Green tea (Camellia sinensis)
- Associated study: Inhibitory effects of commonly used herbal extracts on UGT1A1 enzyme activity.
In Vitro Enzyme Inhibition Experiment
EGCG inhibition of UGT1A1
Inhibition was detected. Cutoff used — > 20% inhibition at the highest tested concentrations
- UGT1A1 40782950
Cell fraction Pooled human liver microsomes -7999662
Concentrations of herbal extracts in screening incubations represent the recommended daily intake (RDI) of each extract dissolved in 53 L, 5.3 L, and 0.53 L. For EGCG, inhibition at the represented RDI was compared with the recommended daily dose (250 mg) of EGCG. The value per dose index (VDI) value was 32.1 L, which is the highest value of all extracts tested, indicating the highest potential for inhibition.
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Precipitant concentrations were estimated from Fig. 2.
0.5 mg/mL
Commercially available
250 µL
30 min
UDPGA
Not available
Not available
25 µM
0.05-500 µg/mL