- Natural product: Goldenseal (Hydrastis canadensis)
- Associated study: Time-Dependent Inhibition of CYP2C19 by Isoquinoline Alkaloids: In Vitro and In Silico Analysis
In Vitro Enzyme Inhibition Experiment
CYP2C19 Inhibition by Berberine
Negligible Inhibition was detected. Cutoff used — TDI ≥1.5 fold decrease compared to preincubation
- CYP2C19 4311137
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed
Results
Table 1
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
1.5 pmol
Commercially available
NADPH regenerating system
1 μM
30 min
NADPH with precipitant