- Natural product: Cat's-claw (Uncaria tomentosa)
- Associated study: Identification of PXR activators from Uncaria tomentosa (Cat’s Claw)
In Vitro Nuclear Receptor Induction Experiment
Mitraphylline Effect on CYP3A4 mRNA Expression
Induction was detected. Cutoff used —
Not available
- CYP3A4 4306811
Cell system PXR-transfected hepatic cells
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
For the analysis of CYP3A4 expression, ~500,000 DPX2 cells were seeded on a 12-well plate and cultured for 24 h. Cells were treated with herbal extracts or chemicals for another 24 h and then washed with PBS. Total RNA of the cells was extracted using TRIzolTM Reagent and 2 µg of them was used for reverse transcription. Quantitative RTPCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems by Thermo Fisher Scientific, Warrington, UK). mRNA levels of CYP3A4 were normalized to Gapdh.
mRNA expression
~500,000 DPX2 cells seeded on a 12-well plate