In Vitro Transporter Kinetics Experiment

R123 Transport Substrate

Transport Activity was detected.  Cutoff used — Unspecified

rhodamine 123

  • P-gp (ABCB1)

Cell system Caco-2 cells

Positive control - verapamil (5 µM) ER of 7.4

Results

Values from Fig 5

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

60,000 cells/well

Transwell

21-23

54-72

Viability and Function

>300 V cm2

Epithelial Voltohmmeter (EVOM2) coupled with a STX2 ‘chopstick’ electrode (World Precision Instruments)

Assay Conditions

180 min

37 deg C

7.4

1 µM, 5 µM