In Vitro Enzyme Inhibition Experiment

rCYP2C9: CBN-warfarin

Inhibition was detected.  Cutoff used — Not specified

(s)-warfarin

cannabinol (cbn)

7-hydroxywarfarin

  • CYP2C9 4309227

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 No

Results

Tables 2, 3, and 4
IC50 preincubation time recorded at 20 min

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

6 pmol

Commercially available

200 μL

20 min

MgCl2
NADPH regenerating system

5 min

NADPH with precipitant