- Natural product: Kratom (Mitragyna speciosa)
- Associated study: Evaluation of In Vitro Absorption, Distribution, Metabolism, and Excretion (ADME) Properties of Mitragynine, 7-Hydroxymitragynine, and Mitraphylline
In Vitro Transporter Kinetics Experiment
Mitragynine p-gp transport kinetics MDCK
No Transport Activity was detected. Cutoff used — ER of 2
- P-gp (ABCB1)
Transfected / siRNA knock-out / injected cells MDCK transfected cells
Results
ER for 10 uM also 1.1
(Table 4)
Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
---|
Experimental Conditions
70 000 cells/well
200 μL
Transwell
2
30–42
>1100 ohm/cm2
TEER measurements and Ly permeability.
120 min
37 deg C
7.4
5,10 uM