In Vitro Enzyme Inhibition Experiment

3A4 Inhibition by Berberine (Testosterone)

Inhibition was detected.  Cutoff used — not specified

testosterone

berberine 19012197

6beta-hydroxytestosterone

  • CYP3A4 4306811

Cell fraction Pooled human liver microsomes -7999662

Results

Estimate from Fig 1 at 20 μM

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

In-house preparation

HHM-247 and HHM-258

15 min

NADPH

10 min

No NADPH with precipitant

1 or 20 μM