Inhibition of CYP2C9 by mitragynine CSV JSON

In Vitro Enzyme Inhibition Experiment

Inhibition of CYP2C9 by mitragynine

Inhibition was detected.  Cutoff used — High probability of interaction if their IC50 values are less than 1 µM. If IC50 values are between 1 and 10 µM, they have moderate possibility in interaction. Low drug-drug interactions if their IC50 values are greater than 10 µM.



  • CYP2C9 4309227

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed


IC50 values from Table 1, Ki values from Table 3

% enzyme inhibition versus precipitant concentration:
Values estimated from Figure 2. In some cases, SEM could not be estimated from the provided figure.

0.02 μM: -5 ± 2%
0.2 μM: 16 ± 3%
2 μM: 20 ± 8%
20 μM: 54 ± 16%
200 μM: See above data

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Mitragynine was isolated in-house. This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.

Commercially available

50 min

NADPH regenerating system

Not available


0.02-200 µM