In Vitro Enzyme Inhibition Experiment

2C19 Inhibition by Hydrastine

Inhibition was detected.  Cutoff used — not specified

mephenytoin, (S)-

hydrastine

4-hydroxymephenytoin, (S)-

  • CYP2C19 4311137

Cell fraction Pooled human liver microsomes -7999662

Results

Estimate from Fig 1 at 20 μM

Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

In-house preparation

HHM-247 and HHM-258

15 min

NADPH

10 min

No NADPH with precipitant

1 or 20 μM