In Vitro Enzyme Inhibition Experiment
Negligible inhibition of CYP3A4 by M. speciosa methanolic extract
Negligible Inhibition was detected. Cutoff used —
- CYP3A4 4306811
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Not available
% enzyme inhibition versus precipitant concentration:
Values estimated from Figure 1d. In some cases, SEM could not be estimated from the provided figure.
0.01 µg/mL: 18 ± Unknown%
0.1 µg/mL: 11 ± 4%
1 µg/mL: 9± 0%
10 µg/mL: 18 ± 4%
100 µg/mL: 50 ± 3%
1000 µg/mL: See above data
|Sample||Compound measured||Value||Measurement||Study sequence||Additional information||N replicates|
M. speciosa methanolic extract was prepared in-house from the leaves of the plant. This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.
The minus-P450 wells (using luciferin-free water) were negative controls.
NADPH regenerating system