In Vitro Transporter Inhibition Experiment

Mitragynine Inhibition

Inhibition was detected.  Cutoff used — Unspecified

rhodamine 123

mitragynine

  • P-gp (ABCB1)

Cell system Caco-2 cells

p<0.05 Values determined from Fig. 5

Results

Results for Mitragynine+R123 were statistically significant (p<0.05) vs R123 alone. Values from Fig. 5

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Preincubated with KRB for 60 min

Cell Culture Conditions

60,000 cells/well

Transwell

21-23

54–72

Viability and Function

>300 V cm2

Epithelial Voltohmmeter (EVOM2) coupled with a STX2 ‘chopstick’ electrode (World Precision Instruments)

Assay Conditions

180 m

37 deg C

7.4

5 µM

5 µM

Department of Forensic Medicine, Johannes Gutenberg University (Mainz, Germany), where it was isolated from Kratom leaves obtained from head&nature

Control Experiment