- Natural product: Kratom (Mitragyna speciosa)
- Associated study: P-glycoprotein interactions of novel psychoactive substances – Stimulation of ATP consumption and transport across Caco-2 monolayers
In Vitro Transporter Inhibition Experiment
Mitragynine Inhibition
Inhibition was detected. Cutoff used —
Unspecified
- P-gp (ABCB1)
Cell system Caco-2 cells
p<0.05 Values determined from Fig. 5
Results
Results for Mitragynine+R123 were statistically significant (p<0.05) vs R123 alone. Values from Fig. 5
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Preincubated with KRB for 60 min
60,000 cells/well
Transwell
21-23
54–72
>300 V cm2
Epithelial Voltohmmeter (EVOM2) coupled with a STX2 ‘chopstick’ electrode (World Precision Instruments)
180 m
37 deg C
7.4
5 µM
5 µM
Department of Forensic Medicine, Johannes Gutenberg University (Mainz, Germany), where it was isolated from Kratom leaves obtained from head&nature