- Natural product: Kratom (Mitragyna speciosa)
- Associated study: Evaluation of selected Malaysian medicinal plants on phase I drug metabolizing enzymes, CYP2C9, CYP2D6 and CYP3A4 activities in vitro
In Vitro Enzyme Inhibition Experiment
POS. CONTROL: Inhibition of CYP2D6 by quinidine
Inhibition was detected. Cutoff used — Not specified.
- CYP2D6 4173631
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Not available
|Sample||Compound measured||Value||Measurement||Study sequence||Additional information||N replicates|
Positive controls sulfaphenazole, quinidine, and ketoconazole were purchased from Sigma Chemicals (St. Louis, USA). This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.
The minus-P450 wells (using luciferin-free water) were negative controls.
NADPH regenerating system