Gou Teng 1/2500 Extracts on CYP3A4 Activity CSV JSON

In Vitro Enzyme Induction Experiment

Gou Teng 1/2500 Extracts on CYP3A4 Activity

Induction was detected.  Cutoff used —

Not available 

midazolam 708298

gou teng extract

1'-hydroxymidazolam

  • CYP3A4 4306811

Cell system DPX2 (HepG2 transfected with PXR)

Results

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Midazolam was utilized as a substrate for CYP3A4 activity assay (Olsen et al., 2016). Briefly, ~250,000 DPX2 cells were seeded on a 24-well plate and cultured for 24 h. After treatment with herbal extracts or chemicals for another 24 h, cells were washed with PBS. Midazolam (50 µM) in fresh medium was added and incubated for 2 h. Afterward, 100 µl medium was collected from each well, and mixed with 100 µl cold acetonitrile. The mixtures were centrifuged at 13,000 rpm for 15 min, and the supernatant was collected for analysis of 1'- OH-MDZ, a CYP3A4-mediated metabolite of midazolam.

Enzyme activity

~250,000 DPX2 cells were seeded on a 24-well plate

UPLC-QTOFMS