In Vitro Transporter Inhibition Experiment

Calcein-AM-THC

Inhibition was detected.  Cutoff used — "The mean accumulation index had to be significantly > 1 (p<0.05) to assess transporter inhibition potency by flow cyotmetry (n = 3 minimum)"

calcein-am

delta-9-tetrahydrocannabinol

  • P-gp (ABCB1)

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Results

Fig 2a.
PSC833 5 μM was used as a positive control

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Assay Conditions

1 hr

37ºC

1.5 μM

50 μM

Sigma-Aldrich