1A2 preincubation without NADPH CSV JSON

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1A2 preincubation without NADPH

Object compound

Precipitant

Object metabolite investigated

Enzymes involved in metabolite pathway

Test system used

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 No

Estimated from fig 2 at 30 mins

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Protein concentration

110-pmol CYP/mg

Test system preparation

Commercially available

Test system lot number

41273

Incubation volume

400 μL

Incubation time

25 min

Co-factors

NADPH
NADPH regenerating system
P450 reductase

Object concentrations tested

100 μM

Precipitant pre-incubation time

0,5,15,30, and 45 min

Precipitant pre-incubation condition

No NADPH with precipitant

Precipitant concentrations tested

75 μM

NOTE