Green tea (Camellia sinensis)
Green Tea Characterization of Material CSV JSON

CoA for Green Tea.


1 . Green Tea Characterization of Material Experiment (id=NPDI-PsGh1w)


Quantities are reported as mass of consistent per mass of green tea leaves.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Material Preparation

200 mg

20 mL


20 mL


For product extraction, 200 mg of sample and 20 mL methanol were added to a 20 mL scintillation vial. Mixtures were shaken overnight at room temperature, filtered, and dried under reduced pressure and stored at -80°C until analysis.

NMR Analysis

JEOL ECA-400 spectrometer

<sup>1</sup>H (proton)

400 MHz

Methanol-d<sub>4</sub> (CD<sub>3</sub>OD)

10 mg/mL

Each sample was resuspended in CD3OD to a concentration of 10 mg/mL. NMR spectra were acquired with a JEOL ECA-400 spectrometer equipped with a high-sensitivity JEOL Royal probe and a 24-slot autosampler. NMR chemical shift values were referenced to residual solvent signals for CD3OD (δH 3.31 ppm).

Mass Spectrometry Analysis

Q Exactive Plus quadrupole-orbitrap mass spectrometer

1 mg/mL

Full scan, high resolution

Electrospray ionization (ESI)

Positive/Negative switching

Waters Acquity UPLC

Water:Acetonitrile (A:B)

95:5 (A:B) for 1 min, then gradient to 0:100 over 20 minutes, holding for 1 min at 0:100

0.3 mL/min

Waters Acquity UPLC BEH C18 1.7 μm, 2.1 × 50 mm

The mass spectrometer was operated in the positive/negative switching ionization mode over a full scan range of m/z 150-2000 with the following settings: capillary voltage, 5 V; capillary temperature, 300 °C; tube lens offset, 35 V; spray voltage, 3.80 kV; sheath gas flow 35 and auxiliary gas flow 20.

Metabolite Quantification




0.1-200 µg/mL



Quantification of the major catechin, phenolic acid, and flavonoid components of the green tea products used 15 calibration standards. LC-MS analysis was conducted as described. Standards were prepared in spectrometric-grade MeOH and diluted in a two-fold dilution series ranging from 0.1-200 μg/mL before injection. A calibration curve was constructed by plotting the area of the selected ion chromatogram for each standard versus nominal concentration. Concentrations of each standard in the extracts were determined by 1/x2 weighted least-squares linear regression.