7-Hydroxymitragynine P-gp Inhibition CSV JSON

Natural product: Kratom (Mitragyna speciosa)
Associated study: Evaluation of In Vitro Absorption, Distribution, Metabolism, and Excretion (ADME) Properties of Mitragynine, 7-Hydroxymitragynine, and Mitraphylline

In Vitro Transporter Inhibition Experiment

7-Hydroxymitragynine P-gp Inhibition

Inhibition was detected. Cutoff used —

EC50 positive control verapamil (22.3± 1.4 μM)

Object compound

calcein-am

Precipitant

7-hydroxymitragynine

Transporters involved

P-gp (ABCB1)

Test system used

Transfected / siRNA knock-out / injected cells MDCK transfected cells

Results

positive control verapamil (22.3± 1.4 μM)

Fig 1S (with exact values in text pg 574)

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Experimental Conditions

Cell Culture Conditions

Density

70 000 cells/well

Protein amount/well or concentration

200 μL

Plate type

Transwell

Days after plating

Passage number

30-42

Viability and Function

TEER

>1100 ohm/cm2

Barrier integrity method

TEER measurements and Ly permeability.

Assay Conditions

Incubation time

120 min

Incubation temperature

37 deg C

Incubation pH

7.4

Object concentrations tested

5 uM

Precipitant concentrations tested

5 uM

Precipitant source

ChromaDex

NOTE

7-Hydroxymitragynine P-gp Inhibition CSV JSON

In Vitro Transporter Inhibition Experiment

Inhibition was detected. Cutoff used — EC50 positive control verapamil (22.3± 1.4 μM)

Results

Experimental Conditions

Inhibition was detected. Cutoff used —

EC50 positive control verapamil (22.3± 1.4 μM)